CLCN7 Knockout cell line (U-2 OS)

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CLCN7 Knockout cell line (U-2 OS) Order

CLCN7 Knockout cell line (U-2 OS)
Catalog#
YKO-H856
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CLCN7 Knockout cell line (U-2 OS)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CLCN7 Knockout cell line（U-2 OS）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
CLCN7
Gene ID
1186
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% McCOY's 5A+10%FBS
Accession
CVCL_E1DR(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript CLCN7-202)
Validation: PCR+Sanger Sequencing
CLCN7 Knockout cell line (U-2 OS) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:U-2 OS		 STR Info (Cell bank)
Cell Line:U-2 OS
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 13131313
D7S820 11121112
D16S539 11121112
VWA 14181418
TH01 69.369.3
AMEL XXXX
TPOX 11121112
CSF1PO 13131313
D12S391 1920
FGA 2020
D2S1338 2024
D21S11 3131
D18S51 1214
D8S1179 1214
D3S1358 1616
D6S1043 1111
PENTAE 1013
D19S433 1515
PENTAD 99
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLCN7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2 OS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLCN7 gene knockout CLCN7 Knockout cell line（U-2 OS） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CLCN7 Knockout cell line (U-2 OS)
CLCN7 Knockout cell line (U-2 OS)
Catalog#
YKO-H856
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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